Cost-effective and robust genotyping using double-mismatch allele-specific quantitative PCR
نویسندگان
چکیده
منابع مشابه
Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping
Customizable endonucleases such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) enable rapid generation of mutant strains at genomic loci of interest in animal models and cell lines. With the accelerated pace of generating mutant alleles, genotyping has become a rate-limiting step...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2019
ISSN: 2045-2322
DOI: 10.1038/s41598-019-38581-z